The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. 00. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Molecular Devices (UK) Ltd. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. The VersaMax reads 96-well microplates. Supplier: Molecular Devices. 003 fmol 0. The 6-well plates were incubated at 37ºC for 9 days before picking. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The equation that allows one to calculate absorbance from % transmittance is. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Janet got excellent results using them and they are only ~$1. Used. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Published Dec 16, 2020 | Updated Jun 22, 2023. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Turn the instrument around so that the back of the instrument is. 306 plate air cal. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 0200-6262. 4 (Molecular Devices cat. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 0. *For Corning UV plates, mean values at 260 nm ranged from 0. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. 96- and 384-well microplates. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. We would like to show you a description here but the site won’t allow us. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 96- and 384-well. M5. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 312 gain calibration failed. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. x. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. Cayman Chemical. to each well. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. x. . White 96-well plate Plate formats. It has a 340-750nm Spectral Range. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. #iD5-STD). 150 µL of deionized water was pipetted into each well of a clean 96-well plate. 9000-0756. Mode Plate Reader. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The picked. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Top and bottom reads are. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). * Note: Programming the washer with a final aspirate height of 4. Users can choose to process all plates within the magazine or select a specific number. BMG Labtech - THERMOstar. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. D. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 40266. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 00 Add to cart; Molecular Devices EMax Microplate Reader $. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Multimodal plate reader: Yes: 25 mm: Yes—Max. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. 046 to 0. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. QuickSync. 90349). The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. Plate and Setup Rack Options Screens with selection of up to four Methods shown. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. x. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Plus 384. Figure 2-23: Bottle holder To install the bottle holder: 1. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Detection cartridge modularity allows for easy addition of new read modes within minutes without. TNF standard curve. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 96-well plates - Filter plates with 0. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. 984. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. These lab workhorses are used in drug discovery. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. for molecular biology. Or scan up to 384 wells at any time to determine the best assay conditions. 96-well microplate compatible Well read area range: D4. Fluorescence Microplate Reader Comparison Chart. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. style. 2. We would like to show you a description here but the site won’t allow us. 17,000 cells. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. • High antibody-binding surface improves the overall performance in. Bowman Dispensers. Published Dec 16, 2020 | Updated Dec 16,. • Remove media from each well using a blue pipette tip being careful as not to remove cells. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. e 3370 Costar 96 Well. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. with Quartz ABS Plastic. VMax . From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . Cell viability and cytoxicity assays. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. Answer. 5 fg per well to 0. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Highly selective for dsDNA over ssDNA or RNA. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. 2. Microplates, cuvettes (via adapter) Detectors. 25, 3. 2 OD at 200 nm; this decreased to 0. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. #E010) Pyroplate 96-well microplate (ACC cat. PURPOSE. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The procedures in this protocol support the 96-well reaction plate. Microplate readers, SpectraMax®, with SpectraDrop. x. With various on-board features and capabilities, the ThermoMax. Top or bottom detection is available with a simple click of a button. Supersedes 1. 96-well) while others have flexibility to. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Next-generation luminescence plate reader. Some will only support one well density (e. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. S. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. Thermomax model provides thermal control and vertical shake, perfect for. 0. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Mix samples in microplate wells by user-programmable Automix feature. Temperature control: 4°C above ambient to 45°C. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 5 fg per well to 0. The plate reader optics are tunable for. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Microplate format. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. Select models. SoftMax Pro GxP Software version 5. Absorbance Linearity: 2. B. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. #C1500) Control standard endotoxin (ACC cat. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. x. No. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. More Product Information. It also integrates with the. validation, maintenance, and repair documentation. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. The soil was amended with either 1. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. x. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. and glow luminescence modes in 96- to 384-well plates. 1% in white 96- and < 0. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. HeLa. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. Eight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. Three low volume 24-well bottom slides. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Artel MVS Bundle DM Std/800TSNB Reader Details. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. Fluorescence Microplate Reader Comparison Chart. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). The CellTiter-Glo assay was used to measure ATP. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Two high-energy xenon flash lamps: Reading capabilities. #SpectraMax L Config) Methods. 4 (Molecular Devices cat. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. The plate reader can measure multiple readouts, including UV and. The CellTiter-Glo assay was used to measure ATP. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 0200-6263. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Place the microplate in the drawer. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. Other technologies such as imaging. Another consideration, says Amouretti, is plate format. 5 second read per plate. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. Working in. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 1. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. x. tools to expand the boundaries of their research. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . May require LS-50B…. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Running both standard spectrophotometer and microplate. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Learn more. They are also sealable using different types of adhesive mats foils or films. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. and the A1 corner of the plate adapter (Figure 1-2). flat-bottom: 50-60 µl. Range of 96, 384-well SBS microplates . The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. • 0. REF (SpectraMax. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Inconsistent results: cannot determine whole well confluence – well after well. Consequently, FLIPR has been used. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Published Dec 16, 2020 | Updated Dec 16, 2020. Back plate and…. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). Materials. Sample by eye within . An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. 384-WELL SBS STERILE MICROPLATE. 96-well-plate-scan-time-2-colors-1-site-well. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Spectrofluorometer from Molecular Devices® provides a flexible environment. x. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Selects either 96-well or 384-well plate for a stand-alone read. Infinite possibilities. the use of a reader calibrated with primary NIST and NMI standards. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Compact designs make these instruments. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. The system features 96, 384,. $14,995. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. 100 fmol 1536-well (8 µL) 0. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. Polypropylene deep well plates, round bottom. When a. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Plate. 25% and 0. Packed 25 plates per…. Component A (2 vials*)Figure 1. Gemini EM Reader scans. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Temperature control range from Ambient +4º to 50ºC. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Reads various kinds of 96-wells plates, and is equipped with shaking function. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. A control well was set up to verify. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. (96- and 384-well with pipetting) 6-384 (96- and 384-well). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. The opaque walls prevent stray light from being detected from neighbouring. ©2022 Molecular Devices - All rights. Uncontrolled Copy for Training and Reference Purposes Only . 5in (46. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Linear, Orbital Slow, and Orbital Fast. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Microplate Readers include the M3, M4, M5, and M5e models. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. 76358-624. Corning. Categories: Plate Readers. 0 . Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Four wash/rinse bottles are included and configurable for both 96- and 384. The device was able to achieve 3σ limits of detection of ca. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Page 1 of 8 . No tools required to assemble. 7°C and the bottom to 36. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Well scanning With well scanning, multiple points within each well can be read, providing a high. High-throughput, automated dynamic light scattering. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. p-Nitroaniline - Catalog No. 500 USD. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc.